ChloroquineV1, Main, Exploration, bibRecord, 002653

Melanin Granules of Retinal Pigment Epithelium are Connected with the Lysosomal Degradation Pathway

Identifieur interne : 002653 ( Main/Exploration ); précédent : 002652; suivant : 002654

Melanin Granules of Retinal Pigment Epithelium are Connected with the Lysosomal Degradation Pathway

Auteurs : Ulrich Schraermeyer [Allemagne] ; Swaantje Peters [Allemagne] ; Gabriele Thumann [Allemagne] ; Norbert Kociok [Allemagne] ; Klaus Heimann [Allemagne]

Source :

Experimental Eye Research [ 0014-4835 ] ; 1999.

RBID : ISTEX:5FD9A56D91A34AE8B5257D9E37A5C77050D56DA5

English descriptors

KwdEn :
- chloroquine, electron microscopy, lipofuscin., lysosome.
Teeft :
- Arrowhead, Asterisk, Chloroquine, Chloroquine treatment, Degradation, Early stage melanosomes, Electron microscopy, Epithelium, Gold granules, Gold particles, Granule, High numbers, Higher numbers, Immature melanosomes, Latex, Latex beads, Latex particles, Lysosomal, Lysosome, Mature melanin granule, Mature melanin granules, Melanin, Melanin granules, Melanogenesis, Melanosome, Melanosomes, Membrane, Outer segments, Phagosomes, Pigment epithelium, Premelanosomes, Residual material, Retina, Retinal pigment epithelium, Schraermeyer, Stieve, Subretinal, Subretinal injection, Subretinal space, Tyrosinase, Vacuole.

Abstract

Abstract: Melanosomes are closely related to lysosomes and lipofuscin granules. This paper indicates the potential involvement of lysosomal degradation processes in retinal pigment epithelial (RPE) cells. RPE cells cultured on Bruch's membrane and choroid were fed with indigestible latex beads. The RPE cells from this preparation were treated with chloroquine to investigate whether membrane swelling, typical for lysosomes under this condition, can be induced in melanosomes. To investigate the fate of indigestible material associated with rod outer segments (ROS), gold-labeled ROS were injected transsclerally into the subretinal space of Long Evans rats using a 32 gauge Hamilton syringe. The degradation of labeled ROS was observed after 5 and 12 days by electron microscopy. The following results were observed. Latex particles fuse with the melanin granules of the RPE. Following chloroquine treatment, the membranes of melanin granules fused, and formed large clusters and vacuoles. Gold granules were detected inside both early stage melanosomes and mature melanin granules of the RPE cells 5 and 12 days following subretinal injection of the labeled ROS. Higher numbers of gold granules were predominantly found in immature melanosomes containing still melanofilaments and in small fused mature melanin granules. In conclusion the effect of chloroquine clearly demonstrates that the melanosomes posses active proton pumps which is typical for lysosomes. In RPE cells stressed by overload with rod outer segments or by ingestion of undegradable material (latex beads, gold particles), fusion of these phagosomes with melanosomes of different maturity is more a general rule than an exception. Therefore, melanosomes are connected to lysosomal pathways in RPE cells.

Url:

https://api.istex.fr/ark:/67375/6H6-PKNQPPLB-C/fulltext.pdf

DOI: 10.1006/exer.1998.0596

Affiliations:

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Le document en format XML

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<term>Electron microscopy</term>
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<term>Higher numbers</term>
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<front><div type="abstract" xml:lang="en">Abstract: Melanosomes are closely related to lysosomes and lipofuscin granules. This paper indicates the potential involvement of lysosomal degradation processes in retinal pigment epithelial (RPE) cells. RPE cells cultured on Bruch's membrane and choroid were fed with indigestible latex beads. The RPE cells from this preparation were treated with chloroquine to investigate whether membrane swelling, typical for lysosomes under this condition, can be induced in melanosomes. To investigate the fate of indigestible material associated with rod outer segments (ROS), gold-labeled ROS were injected transsclerally into the subretinal space of Long Evans rats using a 32 gauge Hamilton syringe. The degradation of labeled ROS was observed after 5 and 12 days by electron microscopy. The following results were observed. Latex particles fuse with the melanin granules of the RPE. Following chloroquine treatment, the membranes of melanin granules fused, and formed large clusters and vacuoles. Gold granules were detected inside both early stage melanosomes and mature melanin granules of the RPE cells 5 and 12 days following subretinal injection of the labeled ROS. Higher numbers of gold granules were predominantly found in immature melanosomes containing still melanofilaments and in small fused mature melanin granules. In conclusion the effect of chloroquine clearly demonstrates that the melanosomes posses active proton pumps which is typical for lysosomes. In RPE cells stressed by overload with rod outer segments or by ingestion of undegradable material (latex beads, gold particles), fusion of these phagosomes with melanosomes of different maturity is more a general rule than an exception. Therefore, melanosomes are connected to lysosomal pathways in RPE cells.</div>
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Melanin Granules of Retinal Pigment Epithelium are Connected with the Lysosomal Degradation Pathway

Melanin Granules of Retinal Pigment Epithelium are Connected with the Lysosomal Degradation Pathway

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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